Effect of IHPLUS® on the germination process of Sorghum bicolor L. (Moench)
Abstract
The objective of this study was to evaluate the effect of the bioproduct IHPLUS® and its immersion times, on the germination process of Sorghum bicolor L. Moench cv. UDG-110. Ten treatments were studied: control (distilled water); and other nine with application of the product in three concentrations (2, 4 and 6 %) and three immersion times (2, 4 and 6 h). The seeds were planted on 5-cm diameter Petri dishes, on support of filter paper moisturized with distilled water. The following indicators were evaluated: germination percentage and value, root length and aerial part length, α-amylase activity and content of proteins and reducing sugars. A completely randomized design was used with four replicas per treatment. The results were processed with the statistical package SPSS® version 15.0. An ANOVA was used and Tukey’s multiple range test was used for mean comparison. The treatments with IHPUS® showed a positive effect
on the germination process. An increase in the germination value was observed in the first 48 h of the essay with the application of the product, as well as higher values in root and aerial part length. There was an increase of the α-amylase activity which was in correspondence with an increase in the concentrations of reducing sugars and total soluble proteins. It is concluded that IHPLUS® is an effective bioproduct to stimulate the germination process of S. bicolor L. Moench cv. UDG-110, with potentialities for the development of agroecological agriculture.
References
2. Babu, Dincy & Balasaravanan, T. Evaluation of the efficiency of plant growth promoting rhizobacteria and its effect on germination of Solanum melongena L. seeds. Int. J. Innov. Res. Sci. Eng. Technol. 6 (1):576-581, 2017. DOI: http://doi.org/10.15680/IJIRSET.2017.0601103.
3. Biswas, S.; Lahiri, P. & Das, S. A study on the role of a close homologue of Bacillus cereus isolated from Metaphire posthumaon germination of gram (Cicer arietinum L.) seeds for its use as biofertilizer. J. Global Biosci. 3 (4):708-713, 2014.
4. Carrillo-Castaňeda, G.; Juárez-Muňos, J. J.; Peralta-Videa, J. R.; Gómez, E.; Tiemann, K. J.; Duarte-Gardea, M. et al. Alfalfa growth promotion by bacteria grown under iron limiting conditions. Adv. Environ. Res. 6 (3):391-399, 2002.
5. Chagas Jr., A. F.; Oliveira, A. G. de; Oliveira, L. A. de; Santos, G. R. dos; Chagas, L. F. B.; Silva, A.L. da S. et al. Production of indole-3-acetic acid by bacillus isolated from different soils. Bulg. J. Agric. Sci. 21 (2):282–287, 2015.
6. Chookietwattana, Kannika & Maneewan, Kedsukon. Screening of efficient halotolerant phosphate solubilizing bacterium and its effect on promoting plant growth under saline conditions. World Appl. Sci. J. 16 (8):1110-1117, 2012.
7. Damam, M.; Kaloori, K.; Gaddam, B. & Kausar, R. Plant growth promoting substances (Phytohormones) produced by rhizobacterial strains isolated from the rhizosphere of medicinal plants. Int. J. Pharm. Sci. Rev. Res. 37 (1):130-136, 2016.
8. Djavanshir, K. & Pourbeik, H. Germination value-a new formula. Silvae Genet. 25 (2):79-83, 1976.
9. García-Martín, D.; Saucedo, O. & Castillo, A. UDG-110. Variedad de sorgo de grano blanco con adaptación tropical. Centro Agrícola. 20 (2):90-94, 1993.
10. Grosu, A. I.; Sicuia, Oana A.; Dobre, A.; Voaides, Cătălina & Cornea, Călina P. Evaluation of some Bacillus spp. strains for the biocontrol of Fusarium graminearum and F. culmorum in wheat. Agric. Agric. Sci. Procedia. 6:559-566, 2015. DOI: http://doi.org/doi:10.1016/j.aaspro.2015.08.085.
11. Higa, T. Effective microorganisms: A biotechnology for mankind. Proceedings of the 1st International Conference on Kyusei Nature Farming. Washington D.C.: USDA. p. 8-14, 1991.
12. ISTA. Rules proposals for the international rules for seed testing. Bassersdorf, Switzerland: International Seed Testing Association, 2014.
13. Jamil, M.; Zeb, S.; Anees, M.; Roohi, A.; Ahmed, I.; Rehman, S. et al. Role of Bacillus licheniformis in phytoremediation of nickel contaminated soil cultivated with rice. Int. J. Phytoremediation. 16 (6):554-571, 2014.
14. Khatab, O. H.; Nasib, M. A. A.; Ghoneimy, E. A.; Abo-Elnasr, A. A.; Hassan, H. A-A.; Hassan, M. Y. A. et al. Role of microorganisms in our life's as ecofrindely and replacement for chemical methods. Int. J. Pharm. Life Sci. 6 (2):4221-4229, 2015.
15. Lambrecht, M.; Okon, Y.; Vande Broek, A. & Vanderleyden, J. Indole-3-acetic acid: a reciprocal signalling molecule in bacteria-plant interactions. Trends Microbiol. 8 (7):298-300, 2000.
16. Lowry, O. H.; Rosebrough, N. J.; Farr, A. L. & Randall, R. J. Protein measurement the Folinphenol reagent. J. Biol. Chem. 193 (1):265-275, 1951.
17. Mahadevamurthy, M.; Channappa, T. M.; Sidappa, M.; Raghupathi, M. S. & Nagaraj, A. K. Isolation of phosphate solubilizing fungi from rhizosphere soil and its effect on seed growth parameters of different crop plants. J. Appl. Biol. Biotechnol. 4 (6):22-26, 2016. DOI: http://doi.org/10.7324/JABB.2016.40604.
18. Miller, G. L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31 (3):426-428, 1959. DOI: http://doi.org/10.1021/ac60147a030.
19. Mohd Din, A. R. J.; Hanapi, S. Z.; Supari, N.; Alam, S. A. M.; Javed, M. A.; Tin, L. C. et al. Germination, seedling growth, amylase and protease activities in Malaysian upland rice seed under microbial inoculation condition. J. Pure Appl. Microbio. 8 (4):2627-2635, 2014.
20. Mohite, B. Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. J. Soil Sci. Plant Nutr. 13 (3):638-649, 2013. DOI: http://dx.doi.org/10.4067/S0718-95162013005000051.
21. Morais, Tamara P. de; Brito, C. H. de; Branda, A. M. & Rezende, W. S. Inoculation of maize with Azospirillum brasilense in the seed furrow. Rev. Ciênc. Agron. 47 (2):290-298, 2016.
22. Olle, Margit. The influence of effective microorganisms on the growth and nitrate content of vegetable transplants. Journal of Advanced Agricultural Technologies. 2 (1):25-28, 2015. DOI: http://doi.org/10.12720/joaat.2.1.25-28.
23. Proietti, Ilaria; Frazzoli, Chiara & Mantovani, A. Exploiting nutritional value of staple foods in the world’s semi-arid areas: risks, benefits, challenges and opportunities of sorghum. Healthcare (Basel). 3 (2):172-193, 2015. DOI: http://doi.org/10.3390/healthcare3020172.
24. Qin, S.; Zhou, W.; Li, Z. & Lyu, D. Effects of rhizobacteria on the respiration and growth of Cerasus sachalinensis Kom. Span. J. Agric. Res. 14 (2):1-13, 2016.
25. Sigarroa, A. Biometría y diseño experimental. La Habana: Editorial Pueblo y Educación, 1985.
26. Soares, Vanessa N.; Radke, Aline K.; Tillmann, Maria Â. A.; Moura, Andréa B. & Schuch, L. O. B. Physiological performance of rice seeds treated with thiamethoxam or rhizobacteria under different temperatures. J. Seed Sci. (Londrina). 36 (2):186-193, 2014. DOI: https://dx.doi.org/10.1590/2317-1545v32n2925.
27. Taiz, L. & Zeiger, E. Fisiología vegetal. 5 ed. Porto Alegre, Brasil: Artmed. 2013.
28. Thakur, A. & Parikh, S. C. Auxin hormone production and plant growth promotion by phosphate solubilizing bacteria of groundnut rhizosphere. Int. J. Innov. Res. Sci. Eng. Technol. 4 (9):8539-8548, 2015. DOI: http://doi.org/10.15680/IJIRSET.2015.0409078.
29. Ullah, F.; Bano, A. & Nosheen, Asia. Effects of plant growth regulators on growth and oil quality of canola (Brassica napus L.) under drought stress. Pak. J. Bot. 44 (6):1873-1880, 2012.
Al enviar un artículo para su publicación en la revista Pastos y Forrajes, el autor garantiza que es propietario de los derechos de explotación de la obra y, en consecuencia, garantiza que puede transferirlos sin ningún tipo de limitación.
En todo caso, responderá por cualquier reclamo que en materia de derechos de autor se pueda presentar, exonerando de cualquier responsabilidad a la revista.
El autor está de acuerdo en ceder a la revista Pastos y Forrajes, de manera no exclusiva, los derechos de explotación sobre la obra antes mencionada (reproducción, distribución, comunicación pública y transformación) por el tiempo que establezca la ley nacional e internacional y sin perjuicio del respeto a los derechos de autoría moral. En virtud de lo anterior, se entiende que la revista adquiere el derecho de reproducción en todas sus modalidades, incluso para inclusión audiovisual; el derecho de comunicación pública, distribución y, en general, cualquier tipo de explotación que de las obras pueda realizarse por cualquier medio conocido o por conocer.
El autor podrá difundir copias de la versión publicada del artículo en el repositorio digital de su institución o en otros repositorios digitales de acceso abierto, bajo la condición de hacer referencia a la fuente original donde fue publicado el artículo, incluyendo un enlace al artículo publicado en la revista.
El autor está de acuerdo con que los usuarios finales de la obra puedan copiar, distribuir, comunicarla públicamente y hacer obras derivadas; bajo las condiciones siguientes: reconocimiento y cita del autor original, no utilizarla con fines comerciales y compartirla bajo la misma licencia.
