AMPLIFICACION DEL MARCADOR MOLECULAR Rrn5 EN ADN EXTRAIDO DE CALLOS DE STYLOSANTHES GUIANENSIS CV. CIAT-184

Abstract

Several genomic DNA isolation protocols as well as the Puregene kit (Gentra System) were
evaluated in lyophilized samples of leaves and callus from Stylosanthes guianensis cv. CIAT-184, in order to obtain optimum samples to amplify, through PCR technique, the Rrn5 ribosomic spacer as possible molecular marker to detect genetic variability induced by in vitro culture. The Zhu et al. protocol (1993) allowed to obtain an average of 500 ng/µL with the purity required for amplifying the molecular marker from 10 ng of DNA. Gel electrophoresis showed a fragment of 250 bp in all samples.