MICROPROPAGACION DE Bromelia pinguin LINDL. (PIÑA RATON)

Abstract

Bromelia pinguin was traditionally used by farmers to enclose the limits of the farms, forming hedgerows which
prevent the entrance or setting of animals. It's natural propagation is slow so, a study was carry out in order to look
for a biotechnological method to propagate it. Apicals bud were used and these were introduced into a MS medium
(Murashige and Skoog, 1962) with vitamins of B5 (Gamborg, Miller y Ojima, 1968) supplemented with 2 mg/l of 6-
BAP and 0,05 mg/l of PBZ (paclobutrazol). This medium was the most adequate for the establishment. This same
was used as multiplication medium without PBZ addition. The rooting was obtained with addition of 0,5 mg/l of ANA
to basal medium. The subcultures were each effected at 28 days with a multiplication factor of 6,2; though in
commercial laboratory scale it was superior to 7. The adaptation of soil and later to the field had a survival rate of
approximately 100 %.